الفهرس 
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Abstract
Hepatitis C virus (HCV) infection is a global health problem. It is the leading
cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma (HCC). The
global distribution of HCV genotypes follows a characteristic geographic pattern.
The interaction between the virus and the host immune system is important in
determining the course of infection and the response to therapy. Polymorphisms at
the interleukin 28B (IL-28B) gene largely influence treatment responses to direct
acting antiviral drugs (DAA) including Sofosbuvir, Daclatasvir with or without
Ribavirin. Single nucleotide polymorphisms (SNPs) in IL-28B gene has been
identified to be associated with HCV treatment response in a genome-wide
association study (GWAS) found that SNP at IL-28B (rs12979860) gene is the
strongest host genetic predictor of sustained virological response (SVR) in
hepatitis C genotype1. Among the three genotypes of IL-28B, CC genotype is
associated with 2 to 3 fold increase in SVR as compared with either CT or TT
genotypes.
Objectives:
This study aimed to assess if there is a relationship between the functional SNP
of IL-28B (rs12979860) gene and HCV treatment response to DAA in an Egyptian
population.
Methods:
This case-control descriptive analytical study included 200 subjects, 100 HCV
patients who received Sofosbtuvir, Dacatasvir ± Ribavirin (50responders&50
non-responders) compared to 100 age and sex matched healthy control subjects.
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The patients were recruited from Tropical clinic in Fayoum University Teaching
Hospital during a period extending from May, 2019 till February, 2020. All
samples were subjected to genomic DNA analysis of SNP of IL-28B gene
(rs12979860) using Real Time PCR.
Results:
The SNP IL-28B gene (rs12979860) was not statistically significantly different
between HCV patients compared to controls (p > 0.05). Moreover, there was a
statistically significant difference (p value<0.05) between the drug resistant HCV
patients and the drug responsive cases as regards CT genotypes.Additionally, there
was a high statistically significant difference in TT genotype (p-value<0.01).
CC represents our reference genotype. The C allele was also higher among
drug responsive patients while the T allele was higher among drug resistant
patients with a high statistically significant difference of p-value <0.01.
Conclusion:
Our study revealed that there is a significant link between IL-28Bgene
(rs12979860) polymorphism and HCV treatment response to DAA. CC genotype is
a good predictor of SVR in chronic hepatitis C genotype 4 Egyptian patients and
this genotype may influence the effect of treatment
Key words:
IL-28Bgene (rs12979860), single nucleotide polymorphisms, sustained virological
response.