الفهرس 
Title : Effect of oxidative stress ondevelopment of in vitro produced camel embryos
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Abstract
The aim of the present study was to evaluate the effect of supplementation of ascorbic acid (first experiment), cysteine (second experiment) and combination of both antioxidants (third experiment) to in vitro culture (IVC) medium on the development of dromedary camel embryos and expression profile of candidate genes regulating embryo quality. Cumulus-oocyte complexes (COCs) were recovered from camel ovaries collected from local abattoir. Good quality COCs were used in the standard protocol of in vitro embryo productionincludingin vitro maturation (IVM) and in vitro fertilization (IVF). The presumptive zygotes were incubated in culture medium with/withoutantioxidant according to the experiment. First experiment: The presumptive zygotes were allocated into four treatments of ascorbic acid, namely; controlgroup (T1), 50 og/ml (T2), 100 og/ml (T3) and 150 og/ml of ascorbic acid (T4).In the second experiment: three cysteine concentrations (T2: 100 æM, T3: 500 æM and T4: 1 mM) were supplemented to IVC medium and compared with control group (T1).The third experiment consisted of four experimental groups; control group without antioxidant (T1), 150 og/ml ascorbic acid (T2), 100 æM cysteine (T3) and a combination of 150 og/ml ascorbic acid and 100 æM cysteine (T4). The relative abundance of b-cell lymphoma 2 (BCL2), catalase (CAT),superoxide dismutase (SOD), glucose transporter 1 (GLUT-1), thioredoxin (TXN) and tumor suppressor protein (P53) genes were assessed using real-time PCR