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Abstract This study was a parallel, randomized clinical trial and evaluated the effect of occlusal reduction on postoperative pain after root canal treatment. The patients were equally randomly assigned to two groups (group A: occlusion reduction; group B: no occlusion reduction). Methods: Forty four patients were included in the study. All included teeth were mandibular molars and premolars with irreversible pulpitis, Preoperative pain and sensitive to percussion and without periapical radiolucency. These teeth were instrumented using rotary Revo-S instruments. For group A, all occlusal contacts on the functional and non-functional cusps as well as on the marginal ridges of the tooth were reduced by 2 mm, while for group B, no modification was done for the occlusal surface of the tooth. Postoperative pain was recorded using a Visual Analogue Scale (VAS) preoperatively and post-instrumentation at 6 hours, 12 hours, 24hours and 48 hours. Also, Post-instrumentation pain was recorded at 12 and 24 hours. All demographic data, post obturation radiographic findings, VAS scores and analgesic intake were statistically analyzed.Results: Results showed that occlusal reduction has no significant difference in pain reduction at 6 hours, 12 hours, 24 hours, and 48 hours post-instrumentation and at 6 hours and 12 hours post-obturation. There was a decrease in the median VAS score through the follow up periods in the two groups with a maximum decrease at 48 hours following root canals instrumentation. There was no significant difference in incidence of analgesic intake between the two groups |