الفهرس 
Assessing and enhancing the efficiency of a novel genetic shuttle vector in mammalian cell lines
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Abstract
Shuttle vectors are vectors that have two promoters and can operate in two different species. Each promoter is compatible with a certain cell line thus mediates the expression of downstream genes in that particular cell line. This is more efficient in comparative gene expression studies and in gene expression in general saving money and time spent on several constructs before conducting the experiments. The shuttle vector used in this study has the human cytomegalovirus (CMV) promoter and OpIE2 promoter to function in mammalian and insect cell lines respectively. The promoters{u2019} activity is measured by the reporter gene EGFP (Enhanced Green Fluorescent Protein). After constructing the vector and assessing the promoters{u2019} activity, a DROP in CMV promoter activity was noticed. The reason behind this reduction was tested and it was found that the 5{u00B4} end of OpIE2 promoter sequence has an inhibitory effect on CMV promoter.This sequence was successfully excised and CMV activity was retained in mammalian cells, while OpIE2 promoter activity was maintained in insect cells