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العنوان
Clinicopathological and molecular studies on ovine theileriosis in Egypt /
الناشر
Mahmoud Akram Ali Eliwa ,
المؤلف
Mahmoud Akram Ali Eliwa
هيئة الاعداد
باحث / Mahmoud Akram Ali Eliwa
مشرف / Mostafa Mahmoud Bashandy Omran
مشرف / Khaled Mohamed Ahmed Mahran
مشرف / Waheed Mohamed Ali Mousa
تاريخ النشر
2021
عدد الصفحات
99 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
3/10/2020
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Clinical Pathology
الفهرس
Only 14 pages are availabe for public view

from 118

from 118

Abstract

Ovine theileriosis is one of the hemoprotazoal disease that is transmitted by tick bites and causes severe economic loss.This study aimed to diagnose sheep theileriosis in 3 regions of Egypt (Cairo, Giza and Al Monofia) by using different clinicopathological, pathological and molecular tools. A total of 152 sheep blood samples were selected randomly from farms and veterinary clinics. The incidence of Theileria infection by microscopic examination of blood smears was 21%, while the incidence by PCR (using universal Theileria primer) was 36.8%. The use of species-specific primers showed a result of 53.6% single infection; Theileria ovis, and 46.4% mixed infection; T. ovis and T. lestoquardi. Depending on the PCR results, the samples were divided into three groups: Theileria negative group, T. ovis group and mixed T. ovis and T. lestoquardi group.The examination of all infected groups did not show any significant changes between them. The hemogram showed significant macrocytic hypochromic anemia, leukopenia, neutropenia, lymphopenia, monocytopenia, eosinopenia and thrombocytopenia in Theileria infected groups in comparison with Theileria negative group. Biochemical analysis showed significant hypoproteinemia, hypoalbuminemia, total and indirect hyperbilirubinemia with elevations of AST and GGT activities and azotemia which was characterized by increase of BUN and creatinine concentrations while non-significant changes were detected in A:G ratio and direct bilirubin concentration