الفهرس 
Title: Residual Microbial Contamination and Biofilm Formation in Poultry Houses
AbstractOnly 14 pages are availabe for public view
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Abstract
In order to ensure healthy environment for poultry, cleaning and disinfectionshouldnot be overlooked throughout production process. Few studies haveexamined the hygienic status of broiler house surfaces, e.g. cooling pads, floorand fan dust prior to and after disinfection. The microbiologicalstatus (water-borne and air-borne microorganisms)in four chicken broiler houseslocated in Giza governorate housing complex was examined throughout the growing period andafter disinfection in this study.Total colony counts, coliforms, pseudomonas, yeast, and fungicounts were conducted. This was followed by isolating and identifying airborne andwaterborne fungal species.Twenty-sevensamples were collected on the followingdays (7th, 21st, and 31st) from the water source and water lines using sponge sticks.Moreover,150 grams of dust samples were collected at different points from fanblades and the floor in different parts of each house. Residual microorganisms werecounted 24 hours after disinfection by collecting 18 surface swabs from water lines,floors, and fans for each house. The microbial load in dust and water samples increasedrapidly as the flock grew. Bacterial colonies were more prevalent at the entrances towater lines than at their ends, the counts from the floor were higher than those from fans. Seven fungal species belonging to five different genera were isolated(Aspergillusflavus (100%), Aspergillus niger (100%), Aspergillusfumigatus (87.5%), Mucor sp. (87.5%), Penicillium sp. (75%), Fusarium sp. (37.5%), while Dematiaceous sp. (25 %)). A total of twenty bacterial isolates were found (from dust, cooling pads, and water); the bacteria are K. pneumoniae, P. aeruginosa, Proteusmirabilis, and E. coli . Following disinfection, all microorganism counts declined significantly