الفهرس 
Metagenome Based Analysis of Aflatoxin Degrading Bacteria in Poultry Farms
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Abstract
Aflatoxicosis is a type of poisoning caused due to the consumption of ubiquitous carcinogens called aflatoxins which are produced by toxigenic Aspergillusspecies and contaminate food and agricultural commodities extensively posing major threats to both human health and livestock productivity,especially in the poultry industry. Several decontamination solutions were proposed, but biodegradation methods seemed to be the most attractive solution owing to its specificity and eco-friendliness. Our study aimed to screen and identify aflatoxin degrading bacteria using both metagenome based methods and traditional methods.Ten bacterial isolates were screened using coumarin as a sole carbon source in media (traditional method), their degradation rates ranged between (25-100%) when tested against AFB1, AFB2, AFG1 & AFG2 using HPLC analysis. Biochemical and molecular identification of these isolates revealed that they belong to four genera; Bacillus (6), Pseudomonas (2), Enterococcus (1) andStenotrophomonas(1)including Pseudomonas fluorescens SZ1 that showed the highest degradation rate (” " ~ " ”100% to all aflatoxins) after incubation at 37 {u00B0}C for 72 hrs. in liquid media. While the Metagenome based analysis (microbiome analysis) of 4 environmental samples (2 soil& 2 feed) associated with variable aflatoxin degradation rates revealed that the major taxa usually associated with AF contamination are Proteobacteria, Firmicutes,Bacteroidetes, Actinobacteria and Cyanobacteria