الفهرس 
Introduction Aim of the WorkOnly 14 pages are availabe for public view
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Abstract
This study was conducted at the Clinical Pathology Department, Women Health Hospital, Assuit University Hospitals during the period from December, 2019 through May, 2021. It was carried out on 285 women, Their age ranged between 17 and 35 years old. They were classified into two groups: group I (Cases): 195 women having history of two or more miscarriages group II (Controls): 90 healthy controls less than 35 years with no history of miscarriage and at least one uncomplicated full-term pregnancy Aim of the study: 1. To assess the relationship between Prothrombin gene and MTHFR gene polymorphisms and recurrent miscarriage. 2. To assess the prevalence of other thrombophilia markers (Homocysteine, Protein C, Protein S and Antithrombin III) in patients with recurrent miscarriage. All study participants were subjected to the following: 1- Complete history taking. 2- Complete clinical examination. 3- Transvaginal ultrasound to exclude any gynecological anatomical abnormality and to exclude pregnancy. 4- Laboratory investigations including; Tests for natural anticoagulants: Protein C. Protein S. Antithrombin III. Lupus anticoagulant test. Anticardiolipin antibodies (IgG and IgM). Homocystiene assay. Real time Polymerase chain reaction (PCR) for detection of Prothrombin gene polymorphism. Real time Polymerase chain reaction (PCR) for detection of MTHFR C677T gene polymorphism. Results: In our study we found that: There was a statistically significant lower level of Protein C in cases with RM 7.2% compared to 1.1% in control group. There was a statistically significant lower level of Protein S in cases with RM 65.6% compared to 7.8% in control group. There was a statistically significant lower level of Anti-Thrombin III in RM group was 9.2% compared to 2.2% in control group. Hyper homocysteinema in cases 10.8% compared to 2.2% in control group. There was a statistically significant higher level of homocysteine in RM group than control group. Also our study showed that Hyper homocysteinema in women with family history of VTE was 33.3% compared to 8.5% among women with no family history of VTE and it was statistically significant. There was statistically significant positive correlation between Protein C and Antithrombin III. Regarding, Prothrombin gene G20210A and MTHFR C677T gene polymorphisms we did not find significant difference between cases with RM and control group. We found that Heteromutant C/T present in 38.1% of cases with hyper homocysteinema and heteromutant T/T present in 61.9% in cases with hyper homocysteinema and it was statistically significant.