الفهرس 
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Abstract
This work was conducted to investigate the hepato-nephroprotective effect of
licorice extract and silymarin against cisplatin hepato- nephrotoxicity in rats.
A total of 72 normal male albino rats body weight ranging from 150-200 gm. The
animals were allowed to acclimatize for one week at the animal house. The rats
were randomly assigned into 6 groups, 12 rats in each group, 12 rats were
randomly selected as the normal control group and fed with commercial balanced
diet. group (1), group (2): licorice extract oral treated group of 2gm/kg body
weight daily for 6 weeks. group (3): silymarin oral treated group 100mg/kg daily
for 6 weeks. group (4): served as hepatotoxic group treated with an injection of
cisplatin intraperitoneally in a dose of 1.5 mg/kg body weight repeated twice a
week for 3 weeks starting from the 4th week of experiment .group (5): received
licorice extract oral treated group of 2gm/kg body weight and injected cisplatin
intraperitoneally in a dose of 1.5 mg/kg body weight repeated twice a week for 3
weeks starting from the 4th week of experiment. group (6): received silymarin oral
treated group 100mg/kg daily for 6 weeks and injected cisplatin intraperitoneally
in a dose of 1.5 mg/kg body weight repeated twice a week for 3 weeks starting
from the 4th week of experiment.
Objective: The present study was carried out to investigate the effect of oral
administration of extract of licorice and silymarin for 3 weeks on hepato -
nephrotoxicity induced to rats by cisplatin (CP). These effects were explored by
measuring relative weight of the liver and kidney , serum levels of liver enzymes;
aspartate aminotransferase (AST), alanine aminotransferase (ALT); albumin,
globulin, total protein, , cholesterol, bilirubin, glucose ,creatinine ,BUN,UA ,and
antioxidant enzymes; reduced glutathione (GSH), , Malondialdehyde (MDA) and
Immunological parameter as TNF- α, IL1- β, B2microglobulin, α-fetoprotein and
CRP level, histopathological and Immunohistochemistry examination of liver and
kidney were also performed.
Abstract
Results: The results showed that the oral administration of licorice extract and
silymarin to intoxicated rats for 6 weeks significantly decreased the elevated serum
levels of liver enzymes (AST, ALT), total bilirubin and increased serum total
protein, albumin, when compared to the corresponding control positive groups.
Levels of antioxidant enzymes glutathione reductase and superoxide dismutase
were significantly increased as compared to the control positive groups.There was
decrease in the elevated CRP, AFP, β2-MG, TNFα and IL-1β when compared to
control positive groups. Histopathological examination of liver and kidney sections
of rats pre-treated with licorice extract and silymarin showed alleviation of
histological degenerative changes caused by cisplatin .the level of caspase-3
cleavage was reduced in pretreated groups compared with cisplatin
Conclusion: The results suggest that, the licorice extract and silymarin induce
potent hepato-nephoprotective and antioxidant effects in CP induced hepatonephrotoxicity in rats. This study recommends that intake of licorice and silymarin
extracts as an herbal may be beneficial for patients who suffer from suffer from
liver and kidney diseases caused by cisplatin usage and oxidative stress.