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العنوان
Surveying Antibiotic Resistance Genes in Some Bacterial Isolates from Local Environment
الناشر
Genetic Engineering and Biotechnology Research Institute
المؤلف
El-Zofzaf Hadear Alaa El Din Abd Allah
هيئة الاعداد
باحث / Hadear Alaa El Din Abd Allah El-Zofzaf
مشرف / Nasser Hussein Rasheed
مشرف / Ahmed Moustafa Hammad
مشرف / Omima Ahmed Khamis
الموضوع
Bacterial
تاريخ النشر
2019
عدد الصفحات
129,P
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الهندسة البيئية
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة مدينة السادات - معهد بحوث الهندسة الوراثية - (Molecular biology and biochemistry
الفهرس
Only 14 pages are availabe for public view

from 129

from 129

Abstract

A total of 90 raw milk cheese sample (n=90) were recovered. Fifty presumptive Enterobacteriaceae spp. were isolated on EMB agar supplemented with cefotaxime (2 μl/ ml) and identified with biochemical tests. The biochemical identification showed that the most prevalent species were E. coli (62%, 31/50) followed by Klebsiella oxytoca (24 %, 12/50), Enterobacter aerogenes (8 %, 4/50) and Yersinia enterocolitica (6 %, 3/50).
The antimicrobial susceptibility testing of Enterobacteriaceae against 15 antimicrobial agents revealed that the most commonly resistance phenotypes were against ampicillin, cefoperazone, cefotaxime, ceftriaxone (100%), amoxicillin-clavulanic acid, cefoxitin and ceftazidime (98%), kanamycin (96%), tetracycline (86%), gentamycin (84%), streptomycin (74%), trimethoprim-sulphamethoxazole (68%), chloramphenicol (66%), and ciprofloxacin (62%). The isolated strains showed the lowest rate of resistance against imipenem (2%). All isolates showed multidrug resistance (MDR) pheno