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Abstract A total of 90 raw milk cheese sample (n=90) were recovered. Fifty presumptive Enterobacteriaceae spp. were isolated on EMB agar supplemented with cefotaxime (2 μl/ ml) and identified with biochemical tests. The biochemical identification showed that the most prevalent species were E. coli (62%, 31/50) followed by Klebsiella oxytoca (24 %, 12/50), Enterobacter aerogenes (8 %, 4/50) and Yersinia enterocolitica (6 %, 3/50). The antimicrobial susceptibility testing of Enterobacteriaceae against 15 antimicrobial agents revealed that the most commonly resistance phenotypes were against ampicillin, cefoperazone, cefotaxime, ceftriaxone (100%), amoxicillin-clavulanic acid, cefoxitin and ceftazidime (98%), kanamycin (96%), tetracycline (86%), gentamycin (84%), streptomycin (74%), trimethoprim-sulphamethoxazole (68%), chloramphenicol (66%), and ciprofloxacin (62%). The isolated strains showed the lowest rate of resistance against imipenem (2%). All isolates showed multidrug resistance (MDR) pheno |