الفهرس 
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Abstract
A liquid chromatography-tandem mass spectrometry based method was validated for the analysis of melamine and cyanuric acid in different food/feed substrates including infant formula. Validation results revealed that, the limit of detection and limit of quantitation for values were 5ng/ml and 10ng/ml for Melamine, 1ng/ml and 5ng/ml for cyanuric acid, the accuracy percentage values were from 94.4% to104% for melamine and from 99.1% to 104% for cyanuric acid, the linearity values were 0.9998 for melamine and 1.0 for cyanuric acid
Tested samples were extracted with 1:1 acetonitrile/water and analysed by High Performance Liquid chromatography-Tandem Mass Spectrometry (HPLC-MS/MS).
Melamine was detected in almost all tested samples (80 samples); the highest concentration observed was 0.93 mg kg−1 and the lowest one was 0.12mg kg−1 melamine, while cyanuric acid was detected in only 4 samples with a range from 0.20 to 0.80mg kg−1 cyanuric acid. The total concentrations of melamine and cyanuric acid in all samples were below the interim standard of 1 mg kg−1 for infant formula products and 2.5 mg kg−1 for other food/feed categories established by WHO.
It can be concluded that, LC-MS/MS technique is a reliable, specific and accurate technique which can be used for qualitative and quantitative analysis of melamine and cyanuric acid in different food/feed categories. Also, the presence of very low concentrations of these two compounds in tested samples indicated the absence of international adulteration of the tested food/feed categories with these compounds.
Keywords: Melamine, Cyanuric acid, LC-MS/MS, Limit of detection, Limit of quantitation, linearity, accuracy, daughter ions, Food, Feed.