الفهرس | Only 14 pages are availabe for public view |
Abstract This study had designed to estimate the antioxidant and hepatoprotective effects of Moringa oleifera and pumpkin seed oil against hepatic toxicity induced by CCL4 in male albino rats. Sixty male albino rats with an initial weight of 110 – 130 g. Before starting the experiment, they were acclimatized for 10 days in animal house. They were randomly divided in plastic cages into 6 groups. 10 rats for each group in each cage. (Group1) as (normal control group) rats received balanced diet only. (Group2) as (Moringa treated group) rats received 0.2 ml of Moringa oleifera seed oil/rat by oral gavage daily for 28 days. (Group3) as (pumpkin treated group) rats received pumpkin seed oil at the dose of 300 mg /kg body weight by oral gavage daily for 28 days. (Group4) as (CCL4-intoxicated group) rats received CCL4 i/p at a dose of 2.5 ml/kg b. wt. dissolved in corn oil (30%) on day 13 and 14 of the experiment. (Group5) (Moringa + CCL4 group) rats received 0.2 ml of Moringa oleifera seed oil/rat by oral gavage daily for 28 days with i/p injection of CCL4 on day 13 and 14 of the experiment. (Group6) as (pumpkin + CCL4 group) rats received pumpkin seed oil at the dose of 300 mg/kg b. wt. by oral gavage daily for 28 days with i/p English Abstract injection of CCL4 on day 13 and 14 of the experiment. The antioxidant and hepatoprotective effects of Moringa oleifera and pumpkin seed oil against hepatic toxicity induced by CCL4 were explored by assessing hematological parameters, liver enzymes levels in serum: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP); total protein, albumin, globulin, A/G ratio, total cholesterol, TG, HDL, LDL, VLDL, and antioxidant enzymes: glutathione reductase (GSH), catalase (CAT), and malondialdehyde (MDA), and histopathological examination of liver tissue. Results: The results revealed that oral administration of Moringa oleifera seed oil and pumpkin seed oil in CCL4 intoxicated rat for 28 days effectively ameliorated hematological parameters, markedly decreased the increased levels of (ALT, AST, ALP, total, direct, and indirect bilirubin and elevated serum levels of total protein, albumin, and globulin and also showed a marked improvement in lipid profile compared with CCL4 intoxicated group. Glutathione reductase, and catalase were markedly increased while malondialdhyde was markedly decreased compared with CCL4 intoxicated group. Liver sections of rats pretreated with Moringa and pumpkin histopathological examination showed favorable changes in degenerative changes created by CCL4. Conclusion: These results advise that oral administration of Moringa oleifera and pumpkin seed oil have potent antioxidant and hepatoprotective effect against CCL4 intoxicated rats. This research recommends that oral administration of moringa and pumpkin seed oil may be favorable for patients with oxidative stress and liver disorder. |