الفهرس 
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Abstract
This study was designed to throw spot upon Staphylococcus isolated from different source (human, animal, food of animal origin) , biochemical and In-Vitro anti-microbial sensitivity for it with special reference to some virulence genes, antibiotic resistance genes and MRSA strains.
So, the present study was performed on a total of 350 samples from different sources from Ismailia governorate.
The incidence of Staphylococcus in the examined samples is 4.3%.
Studying the antibiotic sensitivity test of Staphylococcus spp for 14 different types of antibiotics in lab.
The results of antibiotic sensitivity tests for S.aureus revealed that there is resistance to penicillin, oxacillin, cephalexin and cefaclor by isolates from both human and animal source. All strains isolated from different sources were Sensitive to Chloramphenicol.
Also coagulase negative Staphylococcus isolates were resistance to penicillin.
PCR results revealed that presence of virulence genes (nuc, spa, ica, coa) in only coagulase positive Staphylococcus examined isolates. Also, the presence of antibiotic resistance genes in coagulase positive Staphylococcus examined isolates (mecA, blaZ), but coagulase negative Staphylococcus examined isolates have no virulence genes or antibiotic resistance genes.
In this study there was genetic relatedness among A9, A12 and A13 from (human, animal, food of animal origin respectively) with 94 % genetic similarity which applied by sequencing on PCR product on Ica gene.
On other hand, to investigate the evolutionary relationship between the sequenced gene from our isolates and strains available in the GenBank database, it revealed that there is genetic similarity between human isolate (A9) and swine isolate in china and food isolate in Thiland with 96%.
There is genetic similarity between animal isolate (A12) and human isolate in Germany, USA and china with 96%.
There is genetic similarity between food of animal origin isolate (A13)and human isolate in USA, duck isolate in china with 95%.