الفهرس 
MicroRNAs as biomarkers for detection of liver fibrosis in HCV patients
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Abstract
Chronic hepatitis C virus (HCV) infection causes chronic inflammation and can lead to fibrosis. Egypt has the highest prevalence of HCV in the world, with approximately 13.8% of the population infected. MicroRNAs (miRNAs) are newly discovered small RNAs that repress gene expression and are evolving as clinical predictors for diagnosis and prognosis of human disease. The expression of 20 miRNAs in serum of 47 patients with fibrosis due hepatitis C virus as well as 25 healthy controls was analyzed using qRTPCR real time technique. Also the correlation between the studied miRNAs was analyzed versus each of the clinic-pathological factors collected in serum of patients using T-test. Significant downregulation in serum level of 6 miRNAs was detected (miR-27a, miR-29a, miR-30c, miR-93, mir-106 and miR-191) where 14 were upregulated (miR-17, miR-21, miR-23b, miR-25, mir-34a, miR-122, miR-125b, miR-126, miR-130c, mir-193b, miR-194, miR-199, miR-215 and miR-222) as compared to healthy normal controls. Finally, significance association in the expression level of seven miRNAs (miR-21, miR-122, miR-199, miR-27a, miR-29a, mir-93 and miR-106) with liver fibrosis was observed. In conclusion, the current study showed that seven of the examined miRNAs may be used as potential non-invasive molecular biomarker(s) that are specific to diagnose the liver fibrosis due HCV process