الفهرس 
Molecular detectionof some zoonotic pathogens in rhipicephalus sanguineustick tick
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Abstract
Anaplasma spp. and Ehrlichiaspp. areemerging tick-borne zoonoticbacteriawith expanding reservoirs andvectors than ever. Thecurrent study aimed to molecularly detect and characterizethe Anaplasma species andEhrlichiaspecies in ticks ofRhipicephalus sanguineus sensu stricto (s.s.) infecteddogs in Egypt. For that purpose, ticks were collected from 156 dogs (72 household, 69 kenneled and 15free-roaming). Ticks ofRhipicephalus sanguineus s.s. wereidentified morphologically then molecularly.Anaplasma spp. and Ehrlichiaspp. weredetected in the collected tick pools using PCR targeted16SrRNAof both genera, obtained bands weresequenced and analysed phylogenetically withothersequences recovered from humans and animals and infectionrates werecalculated.Out of the156 collected tick pools, 151weremolecularly positive forRhipicephalus sanguineus. PCR and sequencing for Anaplasmaspp. and Ehrlichiaspp. revealed identification of twobacterial species; A. platys (1.32%) andE. canis(1.98%).A.platyswasidentifiedintwo kenneled dogs tick pools while E. caniswas identified in threetick pools; two fromhousehold dogs and onefrom kenneled one.Moreover, thephylogenetic analysis ofE. canisrevealed genetic relatedness with thoseisolated from human cases