الفهرس 
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Abstract
Dogs serve as hosts for great numbers of parasites like Babesia and Hepatozoon. This study aimed to identify the Babesia vogeli infecting police dogs of different breeds, ages, and sex in police K9 dogs in Egypt using conventional PCR sequencing and phylogenetic analysis followed by the assessment of haematologic analysis, immunological biochemical status of infected dogs. The blood of 242 police dogs was collected for parasitological and molecular diagnosis in addition to hematological, biochemical, and oxidative stress analysis of the blood were performed with gene expression analysis using two genes (gamma interferon [IFN-γ] and tumor necrosis factor alpha [TNF-α]). The 18S rRNA region of canine Babesia species. was successfully amplified, and sequencing data were deposited in GenBank (accession number: MT565474.1), which resembled those of B. vogeli. The results of blood samples revealed that out of the 242 blood samples, 62(25.62%) were positive for B. vogeli infection. Also the study aimed to morpho-molecular identification using 18S rRNA gene (H. canis) and evaluated the blood parameters, biochemical assays of police dogs infested with ticks from k-9 kennels in police academy of Egypt .Ticks and blood samples were c s and morphological identification of ticks. H. canis was detected in neutrophils and monocytes in Giemsa stained blood smears using light microscopy. Serum biochemical assays were done by spectrophotometric methods (urea, creatinine, ALT, AST and serum protein) and blood parameters were examined in the infected blood samples. Polymerase chain reaction (PCR) amplified a " ~ "700 bp fragment of the 18S rRNA gene of Hepatozoon spp. and the DNA products were sequenced. Resulting sequences were compared to each other and with other available representative mammalian carnivore Hepatozoon sequences from GenBank. The present accession number in this study were registered in Egypt for the first time in Genebank as (MW362244.1_ MW362245.1). Sequences of the 18S rRNA gene of H. canis were 99.98% –100.0 % identical to those registers in GenBank. Histopathology form infected freshly died police dogs revealed the appraisal tissues lesions and were described.
Prevalence of Hepatozoon canis was detected in (5%) of dogs by direct micrscopy while it was (10%) by PCR and sequencing. Histopathology revealed that 5 dogs out of 25 died dogs infested with ticks were infected by Hepatoozoon canis, Tick species were identified morphologically as (R. sanguineus).