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Abstract The current study aimed to isolate and identify microbes found in wastewater and test their ability to clean wastewater from pesticides residues. Water samples were collected from El-Khairy drain, El-Beheira Governorate, Egypt. Bacteria and fungi in wastewater samples were isolated and identified individually. Also, pesticides residues were analyzed using the QuEChERS extraction and clean-up method followed by gas chromatography coupled with mass spectrometry (GC-MS) apparatus for the determination of pesticides residues in water samples and growth media. Ten microbes were identified; six isolates were identified as fungi and four bacteria. Pesticides residue analysis revealed the presence of Chlorpyrifos (CPF) as the predominant pesticide in wastewater. Therefore, all microbes were tested for using CPF as a source of energy and clean wastewater out of its residues. The results have proved that the bacterial strain “F: Streptomyces praecox” has shown the greatest activity in cleaning wastewater from CPF insecticide. In conclusion, isolating potentially effective microorganisms from contaminated sites might be important in finding bioremdiators of pesticides in wastewater. |