الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
The present investigation was conducted during two successive seasons of 2017/2018 and 2018/2019 at a private farm, Abou Tisht, Qena governorate and the laboratory of faculty of agriculture, Minia university to examined the influence of humic acid, mixture of three amino acids (tryptophan, methionine and cysteine) and Vit. B1 (thiamine) as well as their interaction treatments on the vegetative growth, yield and volatile oil of anise (Pimpinella anisum, L.) plants. The layout of the field experiment was randomized complete blocks in a split plot design with three replicates. The concentrations of humic acid (0, 2.5, 5 and 10 ml/l) were considered as main plots, while, the concentrations of amino acids and thiamine each at 100, 200 and 400 ppm were occupied the subplots.
The obtained results of the two experimental seasons could be summarized as follows.
Spraying anise plants with humic acid at 2.5, 5.0 and 10 ml/l significantly augmented plant height, stem diameter, branch number and herb dry weight compared to untreated plants in the two seasons. Increasing the concentration of humic acid led to a gradual increase of these traits. The most effective treatments which resulted in the highest values of vegetative growth characters was humic acid at the high concentration (10 ml/l).
B– Yield and yield components
Treating anise plants with humic acid at 2.5, 5 and 10 ml/l) significantly improved the number of umbels/plant, weight of 1000 seeds, fruits yield per plant and per feddan in comparison with check treatment in the first and second seasons. The highest Values of these parameters were obtained from plants received the high concentration of humic acid (10 ml/l) followed by the medium (5.0 ml/l) then by the low concentration (2.5 ml/l).
C– Oil determination:
Oil determination in the fruits (seeds) of anise plants including essential oil percentage, essential oil yield/plant and per feddan were significantly and favorably responded to application of humic acid on anise plants either in the first season or in the second one. In this regard, the values of the oil % and oil yield either per plant or per feddan were gradually increased due to the gradual increase of humic acid from 2.5 to 10 ml/l in the two experimental.
D– Photosynthetic pigments content:
Foliar spray with humic acid at 2.5, 5.0 and 10 ml/l on anise plants led to promoted the content of chlorophyll a in the fresh leaves in the first and second seasons, The highest content of chlorophyll a was recorded from plants treated with the high concentration of humic acid (10 ml/l). Humic acid had significant effect on the content of chlorophyll b in the first season only. Also, it had no significant influence on the content of carotenoids in the two growing seasons.
II- Effect of amino acids and Vit. B1
A– Vegetative growth traits
Treating anise plants with a mixture of amino acids and thiamine (Vit. B1) each at 100, 200 and 400 ppm resulted in a significant increment of the vegetative growth traits i.e. plant height, stem diameter, branch number/plant and herb dry weight compared with untreated plants in the two growing seasons. The Values of these traits were increased gradually due to the gradual raise of the concentration of amino acids or thiamine, in this concern, amino acid treatments were more effective than thiamine in the two seasons.
B– Yield and yield component
Amino acids and thiamine treatments led to significant enhancement of yield components namely, umbels number/plant, weight of 1000 fruits yield per plant and per feddan compared to the check plants in the two experimental seasons. The increase of these parameters was parallel with the raise of the concentration of both amino acids and thiamine from 100 to 400 ppm. In this regard, amino acids were more effective than thiamine. The most effective treatment which gave the highest values of yield components was amino acid mixture at the highest concentration (400 ppm).
C – Oil determination
Essential oil determination in the fruits (seeds) namely, oil percentage and oil yield plant and per fed. were enhanced by spraying anise plants with amino acids and thiamine each at 100, 200 and 400 ppm in the two growing seasons. The highest values of the three determinations were obtained by the high concentration of amino acids (400 ppm).
D– Photosynthetic pigments content:
The content of chlorophyll a was significantly augmented due to amino acids or thiamine treatments in the first and second seasons compared to control, while chlorophyll b content was significantly stimulated by the aforementioned treatments in the first season only the highest contents of chlorophyll a and b were recorded due to amino acid at 400 ppm.
Humic acids and thiamine treatments had no significant influences on carotenoids content in the fresh leaves of anise plants in the two growing seasons.
C-Effect of the interaction
The influence of the interaction treatments between humic acid amino acids mixture and thiamine (Vit. B1) on the vegetative growth, yield, oil determination and photosynthetic pigments of anise plants was significant and beneficial in most cusses. In this regard, the most effective interaction treatment which significantly increased all recorded parameters (except carotenoids content) was spraying anise plants with humic acid at 10 ml/l plus a mixture of amino acids at 400 ppm.
Recommendation
It could be recommended under the experimental and resembling conditions to treating anise plants with humic acid at 10 ml/l and a mixture of amino acids (tryptophan, methionine and cysteine) at 400 ppm in order to obtain the best vegetative growth characters and augment the yield components and enhance the oil yield.