الفهرس 
Studies on multidrug resistant coagulase negative Staphylococcus in chickens
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Abstract
The collected 942 samples from 34 broiler chicken flocks aged and 221 hatchery samples for isolation of Coagulase negative staphylococcus (CoNS). Positive flocks were 15 /34 (34.09%) including 69.23% diseased and 38.95% in apparent healthy. Twenty sex Staphylococcus isolates from 942 samples (2.77%) including 6 liver, 6 kidney, 6 intestine, 4 lung, 2 air-sacs and 2 nasal sinus with rate of 3.82%, 3.82%, 3.82%, 2.55%, 1.27% and 1.27%; respectively. Hatchery samples reveals the isolation of 26 staphylococcus isolates (11.76%) including 12.96% from fertile eggs and 13.79% from dead in shell. Ten isolates were CoNS (38.46%). The obtained isolates were and represented 4.52% out of total samples including 15 S. xylosus, 5 S. scuiri and 2 S. scuiri. Eight S. xylosus was 6 (5.55%) from infertile eggs and 2 (2.29 %) from dead in shell, While the 2 S. scuiri. CoNS isolates showed 100% resistance to Oxytetracycline, Trimethoprime + Sulphamethexole, Calindamycin and Oxacillin. Susceptibility was 100% to Vancomycin and 90% to Enrofloxacin. Multidrug resistance was detected in 2 S.lentus, 5 S. scuiri and 15 S. xylosis; respectively. The detected resistance gens gens are mecA tetK, blaZ , ermC, Kan , icaD in 90, 80, 60 , 90%, 30.0, 30.0 and 0; respectively. One hundred and sixty, 1-day old broiler chicks were grouped into 4 equal groups, at the 10 {u036D}{u036D} {u036A} day birds of groups 1- 3 were s.c inoculated with 0.5 ml containing y 1.5x 10⁸ of S.xylosis, S.scuiri and S.lentus; respectively and group 4 was noninfected control. Clinical signs in infected groups started at 2-3dpi as general signs. Signs disappear in Ciprofloxacillin treated subgroups 24 hr post treatment and lasted to the 7 {u036D} {u036A} day in non treated