الفهرس 
Title : characterization of growth hormone gene (GH) in the Egyptian goat breeds
ContentsOnly 14 pages are availabe for public view
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Abstract
This study assessed the genetic diversity of growth hormone gene (GH) within and between three Egyptian goat breeds (Zaraibi, Barki and Baladi). Polymerase chain reaction and sequencing technique were applied together to generate the full length (2.54 kb) of growth hormone gene. It was found that goat GH gene composed of five exons and four introns and the predicted peptide sequence revealed 217 amino acids corresponding to the coding sequence of GH gene. Different single nucleotide polymorphisms (SNPs) were detected using bioinformatics analysis tools. The results showed nine SNPs common between the three breeds, one SNP common between Zaraibi and Barki breed and five SNPs differentiated between the three breeds. Three amino acid substitutions were detected in exon 3 and exon 4 regions resulting in a damaging effect to the GH protein’s 3D structure. The phylogenetic tree results showed two genetic clusters between the three Egyptian goat breeds and other international breeds and two genetic clusters between the three Egyptian goat breeds and other animals. The results of this work imply the utility of GH gene as a polymorphic marker in genetic selection programs particularly for the Egyptian goat breeds