الفهرس 
Effects of Adding Permeating and Non-Permeating Cryoprotectants on the Quality and Fertility of Buffalo Semen
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Abstract
Recent studies have been conducted to modify semen extenders for protecting buffalo sperm during freezing- thawing process. This study aims to determine (1) the effect of various concentrations of soybean lecithin (SL) as an alternative for egg yolk (EY) in buffalo bull semen extender on pre-freeze and post- thaw sperm quality characteristics. (2) the effectiveness of type and concentration of different non-permeable [egg yolk (EY), soybean lecithin (SL) and cholesterol-loaded cyclodextrins (CLC)], permeable [glycerol (G), di-methyl sulphoxide (DMSO) and ethylene glycol (EG)] and antioxidative [butylated hydroxytoluene (BHT)] cryoprotectants on the functional sperm quality in buffalo bulls pre- and post-freezing extended with egg yolk (TCFYG) and lecithin (TCFGL) - based extenders. (3) The influence of different cryoprotectants on fertility rate of buffalo cows inseminated with frozen-thawed buffalo bull semen extended in TCFYG (control) and TCFGL extenders. Four buffalo bulls belonging to the Abassia Frozen Semen Center, General Organization for Veterinary Services were used in this study. The substitution of egg yolk into TCFG with 1.0% SL and 1.5% SL during equilibration period significantly ameliorated the maintenance of all studied semen characteristics compared to control