![]() | Only 14 pages are availabe for public view |
Abstract The present study is a cross sectional study that comprised a total of 30 Egyptian patients with hepatic cystic echinococcosis (CE) diagnosed by clinical, serological testing (IHA), supported by radiological imaging. Microscopic detection of the protoscolices and fragments of hydatid cyst wall after cyst extraction was done to denote fertility and viability of the cyst with the aim to identify different genotypes of Echinococcus granulosus senso lato that could reflect the dynamic status of these infections in the studied group. Genotyping by a microarray technique applying gold nanoparticle (AuNPs) with silver enhancement as a detection method was used to detect the parasite mitochondrial cytochrome (Cox1) gene from echinococcal cyst fragments. Results showed that 19 out of the 30 cases were G6 (63.3%) and the remaining11cases were G1 (36.7%). There was statistical significance (P<0.005) between the genotype G1 or G6 in relation to patients{u2019}occupation, sero-reactivity, cyst staging by ultrasound as well as multiple organ involvement. It was concluded that G6 genotype was the predominant genotype in Egyptian patients enrolled in this study. The study recommended the use of Gold nanoparticle- based DNA microarray with silver enhancement detection on microarray as a very sensitive and cost-effective molecular method for genotyping of hydatid disease on a large scale |