الفهرس 
Utilization of nanoparticles in extraction procedures for therapeutic drug monitoring
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Abstract
Analytical chemistry is a science that is directed towards creating new knowledge so that chemical analysis can be improved to respond to increasing new demands. Without analytical chemistry we could not make any important decisions about choosing the correct medical treatment for patients. Thus, analytical methods should be accurate and specific to measure plasma drug concentration during the course of treatment which is a part of therapeutic drug monitoring process. Sample clean-up is a crucial step in the analytical procedures. As long as the extract is clean, accurate information about the drug concentration can be obtained using the suitable analytical assay. Nevertheless, current sample preparation techniques suffer from limitations, including matrix interference and intensive sample preparation. In this thesis, a piece of integrated work including modeling, synthesis, analysis, optimization and application to clinical setting was manifested. In this work, a novel solid phase extraction technique using magnetic molecularly imprinted nanoparticles (MMI-SPE) is proposed for extraction of an antiepileptic drug; Levetiracetam and in another case the antileukemic agent; 6-Mercaptopurine and its active metabolite Thioguanine from human plasma. A computational approach is implemented to screen the functional monomers and polymerization solvents to provide a suitable design for the synthesized molecular imprinted polymers. Different analysis techniques and re-binding experiments were performed to characterize the magnetic molecularly imprinted nanoparticles, as well as to identify optimal conditions for the extraction process. Adsorption isotherms and kinetics were studied in detail. A validated HPLC assay was introduced for the quantitation of levetiracetam in plasma after extraction while chromatographic separation of the extracted 6-Mercaptopurine and its active metabolite Thioguanine was carried out using a validated LC-MS/MS assay