الفهرس 
Title : Biophysical evaluation of alfa lipoic acid as radio protector in nuclear cardiology
AbstractOnly 14 pages are availabe for public view
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Abstract
Background: SPECT MPI (Single photon emission computed tomography myocardial perfusion imaging) is an essential tool for diagnosis of cardiovascular disease, but it also involves considerable exposure to ionizing radiation. Objective: The present study was designed to determine the radioprotective potential of lipoic acid free and nano-capsule against 99mTc-MIBI - induced injury in cardiovascular tissue. Methods: The radioprotective ability was assessed by blood count, histopathology and heart enzymes in different groups of mice. Hearts of mice from all groups were dissected and prepared for oxidative stress analysis (superoxide dismutase (SOD) and malondialdehyde (MDA). Furthermore levels of DNA damage in heart and bone marrow cells were evaluated by alkaline comet assay technique. The same measurements were estimated after treating the mice with lipoic acid. Results: Results of mice injected by radiopharmaceutics compared with control group showed a significant depression in the count of WBC (white blood cells) by about 40 % at 24 & 72hrs postradiopharmaceutical administration and moreover decrease in platelets count by 27% at 72hrs postradiopharmaceutical administration. Radiation caused also DROP in SOD (Super Oxide Dismutase) and increase in activity of heart enzymes and level of MDA (Malondialdehyde). Additionally, histopathological observation was characterized by focal necrosis of cardiac myocytes. Moreover, 99mTc-MIBI induced DNA damage as can be inferred from a significant increase in % DNA in tail. Nevertheless, pretreatment with free and lipoic acid nano-capsules (LANC’s) prevented the reduction induced in WBCs and platelets and improved their counts significantly. Conversely pretreatment with lipoic acid free and nano-capsule significantly increased the activity of SOD and decreased the level of MDA and therefore protected the cardiovascular tissues and reduced DNA strand-break, consequently and enhanced the body weight of the mice