الفهرس 
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Abstract
Cryopreservation is a lab technology where cells, tissues, or any other biological component predisposed to injurious insult resulting from disorganized chemical reactions are preserved by a cooling process to very low temperatures. Cryopreservation technology approach low temperature grades in order to prevent damage caused by formation of ice crystals all the way through the freezing process. Various research studies displayed and revealed evidence that embryos frozen and stored implementing slow-freezing technology results have more favorable IVF outcomes more than fresh embryos. Osmotic pulling out of a very large percentage of intracellular water before the cooling process, and not the permeation aspect of cryoprotectant in the cell, is a cornerstone factor for an efficient vitrification lab technique. Vitrification lab technique is simple, and does not require expensive freezing tools, and relies mainly on the insertion of the embryo in a very small volume of vitrification medium that must be cooled at extreme rates not obtainable in classic enclosed cryostorage devices. Embryonic quality is chiefly assessed by microscopy using meticulous time points implementing a morphology system for grading. These usually enhance the conception rates. There is research proof for the possible value of blastocoelic fluid reduction before the vitrification process. Blastocelic maturity at the time point of lab vitrification is a corner stone factor affecting ICSI outcomes .Significant differences in post-warming re-expansion, cell proliferation, and DNA damage have been displayed between expanded blastocysts undergoing artificial collapse before lab vitrification using the laser and revealing that the laser pulse causing minimal cell damage. Artificial collapse of embryos before vitrification is a fairly new technique for enhancing embryonic viability after vitrification. The theoretical basis for implementing this lab technique before starting the vitrification process is to avoid ice crystal damage by decreasing fluid within the embryonic cavity. In the current research study the laser artificial shrinkage group displayed 75.3٪ retrieval of high quality blastocysts after warming and needle AS showed 62.6٪ and there was a statistical significant difference between both groups p value <0.001. Additionally as regards biochemical, clinical pregnancy and implantation rates, there was a statistical significance difference between both groups regarding positive β-hcg, clinical pregnancy, implantation, baby take home rate with p values = 0.007, 0.004, 0.005, 0.012, consecutively.