الفهرس 
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Abstract
Piroplasmosis is caused by tick-borne hemoprotozoans of the genera Babesia and Theileria and is the most prevalent in tropical and subtropical countries, causing a major economic impact worldwide.In this study, 266 tick specimens were collected from 121 infested (buffalo and cattle) from both Egypt and Libya and 174 from 580 infested sheep and goats from Libya. After examination of the specimens by light microscopy and PCR, they were identified as Rhipicephalus annulatus from bovines, Hyalomma marginatum and Rhipicephalus bursa from sheep and goats. Out of 230 (cattle and buffaloes) examined for tick infestation, 55/230 (23.9%) were found infested with one species of hard ticksin Egypt. The overall prevalence of tick infestation in cattle was 66/140 (47%), while in sheep and goats was 580/980 (59%) at (Al Gabal al Akhdar) in Libya. In the present study the blood samples were collected from 314 buffalo and cattle in Egypt, while 343 were from sheep and goats in Libya, the samples were examined by Giemsa stain. The results showed the overall prevalence of piroplasma in cattle and buffaloes was 45/209 (21.5%) in Egypt, while in cattle was 29/105 (27.6%), 116/372 (31%) in sheep, and 13/62 (21%) in goats in Libya. Molecular identification was done for both tick and piroplasma samples. The comparison between the morphological and molecular identification, the molecular identification proves that it will help and improve the disease diagnosis when choosing a good genetic marker.