الفهرس 
Title: Comparison between zeta potential and hyalurenan binding methods to improve sperm selection for intracytoplasmic sperm injection ICSI
ContentsOnly 14 pages are availabe for public view
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Abstract
At present, sperm selection for ICSI only depends on morphology and motility, but these parameters may not be relevant the chromatin integrity. So sperm selection based on sperm functional characterized has been suggested. Thus, aim of this study was the comparison between two sperm selection method, HA binding and Zeta method, to select spermatozoa with normal morphology and intact chromatin. Methods: Semen samples from 150 infertile couples referring to Nile Center For IVF were assessed during this study. Semen analysis was carried out according to WHO criteria. Semen divided in 3 groups. In 2 groups Zeta method and HA binding applied to select spermatozoa and 1 group as control. Sperm morphology, motility and DNA fragmentation were assessed by papanicolaou staining, chromomycin A3 (CMA3) staining, and SCD test, respectively. Result: Both HA binding assay and Zeta method are efficient to select sperm with normal morphology and motility. But in term of DNA fragmentation Zeta method appear to be more efficient to select sperm with low DNA fragmentation. Conclusion: The results of this study suggest that these sperm selection method can select spermatozoa with normal morphology and protamine deficiency and can use of selected sperm in ICSI. But Zeta method may be more efficient to select sperm with low DNA fragmentation. In patient with high DNA fragmentation, Zeta method can be useful to select spermatozoa for ICSI