الفهرس 
المجموعات الفرعية لخلايا الدم البيضاء أحادية النواة
Differential recruitment to inflammation and hepatic fibrosis in chronic hepatitis C patients /Only 14 pages are availabe for public view
 |  | from | 142 |  |  |
| | | from | 142 | | |
Abstract
Background : chronic liver disease is a worldwide common pathology characterized by inflammatory and fibrotic processes that may lead to progressive evolution from chronic hepatitis to cirrhosis. Peripheral blood monocytes may play an important role in the pathogenesis and resolution of liver fibrosis. These cells may offer new approaches for better understanding the pathogenesis of liver fibrosis. Aim of the work: We aim to define the proportion of circulating monocyte subsets with hematopoietic origin in peripheral blood and to establish whether these circulating peripheral blood monocyte subsets are differentially recruited in patients with HCV related chronic liver disease. The potential role of these subsets as non invasive biomarker of liver fibrosis in patients with HCV related chronic liver disease was also investigated. Subjects and Methods: Sixty patients with HCV induced chronic liver disease were classified according to the stage of liver fibrosis after METAVIR score into 4 groups, patients with stages F1, F2, F3 and F4 liver fibrosis, 15 patients each; 15 healthy subjects served as normal controls. Flowcytometric analysis for immunophenotypic characterization for identification of circulating levels of peripheral blood monocytes subsets and CCR2+ve cells in different groups studied was carried out using monoclonal antibodies anti-CD45, anti-CD14, anti-CD16, anti-collagen type I and anti-CCR2, respectively. Determination of serum levels of MCP-1 and SAP in different groups studied was also conducted using ELISA.Results: Data demonstrated a significant down regulation (p< 0.01) in the proportion of classical monocytes subset (CD45+ve, CD14+ve and CD16-ve) in conjunction with a significant up regulation in the proportion of both the non-classical monocytes (CD45+ve, CD14+ve and CD16+ve) (p< 0.01) and monocytes producing collagen (CD45+ve, CD14+ve, CD16+ve and ColI+ve) (p< 0.01) subsets in patients with chronic hepatitis C related liver disease compared to healthy subjects. Data also revealed a significant marked increase in the circulating levels of MCP-1 (p< 0.01) and the proportion of monocytes expressing CCR2 (p< 0.01) along with a significant decrease in the circulating SAP levels (p< 0.01) in different groups of patients compared to healthy subjects