الفهرس 
Immunohistochemical Expression of Cancer Stem Cell Markers (ABCG2 and CD44) in Ameloblastoma, Ameloblastic Carcinoma, Keratocystic Odontogenic Tumor in Comparison to Tooth Follicle (In vitro study)
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Abstract
Odontogenic lesions such as AB and KOT are benign but have aggressive and invasive behavior with high recurrence rates as well which remains the main obstacle for different treatment modalities. CSCs are a small group of cells that present in many neoplasms and they are responsible for their aggressiveness and relapse after the treatment. The current study evaluated immunohistochemical expression of ABCG2 and CD44 (CSC markers) in AB, ABC, KOT and tooth follicle of permanent teeth. To compare the expression of these two markers and correlate the obtained results with the biological behavior of such lesions. The study was performed using thirty seven archival cases of AB (15 cases), ABC, KOT (10 cases each) and tooth follicle of permanent teeth (2 cases). These cases were stained using H&E for histological examination. Indirect biotin free system/HRP-conjugated secondary antibody was used for immunostaining by anti-ABCG2 and anti-CD44 antibodies As regard immunohistochemical evaluation, the stained sections were examined by ordinary light microscope. The area percentage of the positive ABCG2 and CD44 immunoreactions was measured by image analyzer computer system using the software Leica Qwin 500. All the obtained data were statistically evaluated; comparisons among the used lesions were done using ANOVA test, this was followed by Tukey{u2019}s post hoc test when the difference was found to be significant