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Abstract Aljabal Alakhdar or the evergreen mountain (EGM) located in the northeastern Libya is the most vegetated part in the country has the richest vegetation and the highest number of plant species known from Libya. Soil and root fragments from the rhizosphere of Juniperus phoenicea and rhamnus lycioides L. were sampled in July 2016 from 16 locations in Aljabal Alakhdar forestry. spores were collected from the soil samples using the wet sieving technique, counted and morphologically examined for size, color and shape. Spore counts in all soil samples from R. lycioidesL rhizosphere ranged between 29 and 188 spores. g-1 soil with a maximum recorded in samples from Al-Hamama and a minimum in the soil from Aldercya. Morphological spore characteristics of 31 isolated AMF relate them to 11 morphospecies affiliating to 8 genera of 5 families of gGlomeromycota namely Gigspora, claroideoglomus, glomus, archaeospora, entrophospora, funneliformis, rhizophagus and scutellospora with the highest relative abundance (RA) of 38.7% for claroideoglomus eutunicatum followed by gigsporasp. and Entrophospora infrequens with RA of 12.9%. Entrophospora infrequens, Glomus epigaeus and Rhizophagus intraradices were the least encountered in all locations with RA of as low as 3.2%. The rhizosphere of J. phoenicea harbored a maximum number of 188 spores. g-1 soil in Al-Hamama and as high as 148 spores. g-1 soil in Wadi Elkouf location, however, few spores (4 and 6 spores. g-1 soil) were counted in the rhizosphere soil of this tree in Alhunaya and Susah |