الفهرس 
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Abstract
The pathogens that are frequently isolated from wound infections are varied from Gram-positive strains with common prevalence of S. aureus, and Gram-negative isolates including, P. aerugnosa, Klebsiella spp. , Proteus spp., and E. coli.
Therefore, the current study is aimed to identify Staphylococci species isolated from a group of Egyptian patients with different types of wound infections in MUH, detect the antimicrobial resistance pattern and multi-drug resistance profile of all Gram-positive and Gram-negative isolates, and molecular studying of different virulence genes (hla, sea, icaA, and fnbA) of some isolated S. aureus. Also, this study investigates the prevalence of these genes in MRSA, MSSA, and VRSA.
Samples were collected from the patients who attended the Department of Plastic and Reconstructive Surgery in MUH. Samples were obtained from various wounds types including burn wounds, surgical wounds from different anatomical sites, and abscesses and ulcers. Out of 120 samples, 170 isolates were identified. Poly-bacterial infections were determined in 66 (55%) samples while mono-bacterial infections were detected in 54 (45%) samples. S. aureus showed superior sensitivity to vancomycin (85.3%). CoNS were more sensitive to gentamicin with rate of 92.3%. Both S. aureus and CoNS showed high resistance to piperacillin. All isolates elucidated MDR profile.
PCR results show that sea was the most predominant in 72.9% of the isolates. icaA was found in 49.2% of the isolates, followed by hla with rate of 37.3% , and fnbA with rate of 13.6%. sea was the commonest virulence gene among MRSA and VRSA isolates (62.9% and 62.5%, respectively). However, sea and icaA were the commonest genes among MSSA isolates (80%).