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العنوان
Using molecular biology techniques for nematode resistance in sugar beet =
المؤلف
Mohamed, El-Shaimaa Abdelmowla El-Sayed.
هيئة الاعداد
باحث / الشيماء عبد المولى السيد محمد
مشرف / ابراهيم محمد عبده جوهر
مشرف / نادر رجب عبد السلام محمد
مشرف / أميرة فتح الله محمد زيتون
الموضوع
Sugar beet - pesticide.
تاريخ النشر
2021.
عدد الصفحات
x,127,5p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
28/2/2021
مكان الإجازة
جامعة الاسكندريه - كلية الزراعة ساباباشا - النبات الزراعى
الفهرس
Only 14 pages are availabe for public view

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from 149

Abstract

Sugar beet (Beta vulgaris subsp. vulgaris) is the most economically valuable crop species in the order Caryophyllales, B. vulgaris subsp. the genus Beta L. of the family Amaranthaceae (formerly, Chenopodiaceous). Sugar beet is considered one of the most well-known sugar crops. It is a temperate crop; though, it can be cultivated in a wide range of climatic conditions.
In Egypt, Sugar beet, Beta vulgaris L. is deliberated as the first sugar crop cultivated in 954.248 feddans contributing with 62.2% of sugar production. In intensive commercial production, where sequential cropping of one susceptible crop after another is practiced, lack of effective root-knot nematodes management strategies has led to total crop failure. Furthermore, problems associated with nematicides application occur, and nematode management mostly relies on the availability and use of adequate sugar beet varieties. It is obvious that farmers need clear information on the host status of the available sugar beet varieties.
The objective of this study was to establish a rapid and effective screening procedure to detect numbers of sugar beet genotypes with resistance to root-knot nematodes through modified conventional susceptibility assessments confirmed by customizing of molecular markers as respective detection platforms, i.e. markers based on single nucleotide polymorphisms (SNPs). In addition, nanotechnology was employed experimentally to assist a given susceptible sugar beet variety to combat RKN infestation.
Therefore, three outdoors pots experiments were carried out at Sabahia Research Station in the ordinary sugar beet growing season of Egypt at autumn of 2018 and 2019, one was carried out for short-run period (60 days) to assess host suitability of sugar beet genotypes for root-knot nematode, Meloidogyne incognita according to adapted quantitative scheme for assignment of Canto-Saenz’s host suitability (resistance) designations, whereas the two other experiments were carried out in full growing season of sugar beet (180 days).
One of them was conducted to determine the susceptibility or tolerance degree of screened sugar beet varieties by modified host parasite index (MHPI) and the second was carried out to evaluate quantitative and qualitative effects of different concentrations of nanoparticles (Ag-NPs) on the pathogenicity and reproductive of M. incognita infecting susceptible sugar beet variety, Polat.