الفهرس 
Anatomy Physiology of Cornea and Healing ProcessOnly 14 pages are availabe for public view
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Abstract
Summary This study was carried out over a period of 12 months, from January 2021 to December 2021 aiming to evaluate the accuracy of impression cytology smear light microscopy examination as a rapid noninvasive technique in diagnosis of microbial keratitis. The impression cytology smear was collected form suspected microbial keratitis from the Cornea Unit of Ophthalomolgy Department –Faculty of Medicine- Tanta University. The impression cytology smear was taken from 80 cases suspected clinically as microbial keratitis with age ranged from 17 to 81 years with mean SD (43.55+- 15.92). (60%) were males and (40%) were females. The risk factors were (30%) contact lens uers, trauma was (37.5%), immunocompromissed were (15%), eye drops sbuse were (12.5%) and postocular surgery were (5%). With clinical examination (25%) were suspected as pure fungal keratitis, (32.5%) were suspected as pure bacterial keratitis, (32.5%) were suspected as mixed keratitis and (10%) were suspected as acanthamoebic keratitis. The impression cytology corneal smear was collected from all study cases and transported in BHI to the Microbiology department laboratory of Faculty of Medicine of Tanta University to be examined with light microscopy and cultured. Every sample was divided part for staining in 3 slides the first stained with Gram stain to detect bacteria, the second stained with lactophenol cotton blue stain to detect fungi and the third as wet film (no stain) to detect acanthamoeba. The other part of the sample was cultures on Blood agar, MaCconkey’s and Sabaroud’s dextrose agar cultures. The results of impression cytology were (37.5%) with lactophenol cotton blue stain and