الفهرس 
Prevalence and antimicrobial resistance assessment of some pathogens in bulk tank milk and dairy farm environment in Egypt
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Abstract
A total of 775 samples of which 75 were BTM and 700 of farm environmental samples were collected from 5 dairy farms in Kafr-El-sheikh Governorate and Alexandria road, Egypt. Three Governmental farms (A, B and E) and 2 private farms (C and D). The collected samples were examined for the hygienic quality of BTM, the prevalence of some food-borne pathogens and for the antimicrobial resistance of isolated pathogens. The results revealed that the mean values of APC in BTM samples of farms A, B and E were 6.6{u00D7}10⁵±2.4{u00D7}10⁴, 2.7{u00D7}10⁵±3.1{u00D7}10⁴ & 7.4{u00D7}10⁴ ±4.7{u00D7}10³ cfu/ml, respectively, while in private farms C and D were 4.7{u00D7}104 ±3.7{u00D7}103 and 1.2{u00D7}104 ±1.7{u00D7}103 cfu/ml, respectively. The mean values of coliforms in BTM samples of farms A, B and E were 1.6{u00D7}10⁴ ±1.7{u00D7}10³, 1.4{u00D7}10⁴ ±2.9{u00D7}10³ and 3{u00D7}10³ ±1.4{u00D7}10² MPN/ml, respectively, while in farms C and D were 1.3{u00D7}10³ ±3.1{u00D7}10² and 3.8{u00D7}10² ±1.4{u00D7}10² MPN/ml, respectively. The mean values of Staphylococci counts in farms A, B and E were 6.4{u00D7}10⁴ ±1.6{u00D7}10³, 4.2{u00D7}10⁴ ±2.2{u00D7}10³ and 3.5{u00D7}10³ ±4.1{u00D7}10² cfu/ml, respectively, while the count in farms C and D were 2.1{u00D7}10³ ±2.5{u00D7}10² and 1.7{u00D7}10³ ±3.8{u00D7}10² cfu/ml, respectively. The mean counts of SCCs/ml in BTM samples of farms A, B and E were 603{u00D7}10³±310{u00D7}10², 396{u00D7}10³ ±163{u00D7}10² and 169{u00D7}10³ ±140{u00D7}10² cells/ml, respectively, while in farms C and D were 174{u00D7}10³ ±146{u00D7}10² and 142{u00D7}10³ ±96{u00D7}10² cells/ml, respectively. Testing of BTM for quality will provide producers with valuable information concerning the status of health and sanitation in their herds and help to produce high quality BTM. The examination of BTM and dairy farms environment for prevalence of pathogens using PCR technique indicated that farms A and B were positive for all four concerned pathogens (S. aureus, Salmonella spp., E. coli and L. monocytogenes), farm E was positive for 3 pathogens (S. aureus, E. coli and L. monocytogenes), while only S. aureus and E. coli were isolated from farms C and D. Testing of isolated pathogens against 17 antimicrobial agents revealed resistance and sensitivity of these pathogens in various percentages. The hygienic as well as the public health significance of obtained were discussed