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العنوان
Genetic merits of fertility in Egyptian goats /
الناشر
Samira Hamed Sayed Ahmed ,
المؤلف
Samira Hamed Sayed Ahmed
هيئة الاعداد
باحث / Samira Hamed Sayed Ahmed
مشرف / Said Zaki Mostafa
مشرف / Eman Moawad Gouda
مناقش / Said Zaki Mostafa
تاريخ النشر
2016
عدد الصفحات
163 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
1/3/2017
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Biochemistry and Chemistry of Nutrition
الفهرس
Only 14 pages are availabe for public view

from 187

from 187

Abstract

Aiming to estimate the genotypic and allelic frequencies of FSHÝ and FSHR genes in four Egyptian goat breeds in relation to fertility traits, DNA was isolated from whole blood of 160 animals of Baladi, Parqi, Damascus and Zaraibi goat breeds. PCR-SSCP and sequencing for FSHÝ Exon1 revealed five genotypes and several SNPs with significant positive effect of AA genotype on litter size. Two genotypes and one SNP in restriction site of the enzyme at Intron2, Exon3 locus of FSHÝ gene using PCR- RFLP technique were obtained. Polymorphism using PCR- SSCP of FSHR 5{u2019} regulatory region revealed three genotypes and two SNPs, with AA and BB genotypes had greater litter size in Baladi and Parqi does respectively. While, exon10 of FSHR gene had only one genotype indicating homozygosity of this locus among studied breeds. These results revealed that FSHÝ and FSHR genes could be potential candidate genetic markers for improving goat breeding