الفهرس 
The effect of bone marrow derived mesenchymal stem cells treatment on healing of experimental burn injury in rats: possible role of platelet rich plasma
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Abstract
Background: Burn injury is a common cause of morbidity and mortality. It is a serious injury, progressive in nature, causing many of effects far beyond its wounds. Bone marrow derived mesenchymal stem cells (BMMSCs) have shown great potential in cell therapy of wound healing. Platelet-rich plasma (PRP) with increased concentrations of growth factors play an essential role in the complex processes of wound healing and tissue regeneration. This work was designed to study the effect of BM-MSCs and PRP on healing of experimental burn injury, to investigate their mechanism of action and study the effect of PRP on BM- MSCs proliferation in vitro Methods: This work included: an in vivo study and an in vitro study. The in vivo study included seventy two rats which were divided equally into six groups: healthy control group, burn group, burn group that received MSCs only, burn group that received PRP only, burn group that received both MSCs and PRP and burn group that received MSCs pretreated with PRP. On the 7th and 20th day of the injury, histopathological examination of skin tissues, immunohistochemical detection of Platelet-derived growth factor (PDGF), gene expression of Matrix metalloproteinasesl (MMP1), Tissue inhibitors of Matrix metalloproteinases-2 (TIMP-2), angiopoietin 1 (Ang 1) & angiopoietins 2 (Ang 2), and Vimentin in skin tissue by RT-PCR, and estimation of serum of transforming growth factors- beta (TGF-Ý), Tumor necrosis factor- alpha (TNFÜ) and Interleukin-10 (IL-10) by ELISA were performed in all groups. The in vitro study included two groups of cells, the first group included MSCs and the second one included MSCs treated with PRP. Estimation of TGF-Ý by ELISA and cell proliferation assay were performed for the two in vitro groups