الفهرس 
Further insights into the diversity of coagulase positive staphylococci : Special consideration to coagulase gene polymorphism and reaction
AbstractOnly 14 pages are availabe for public view
 |  | from | 154 |  |  |
| | | from | 154 | | |
Abstract
In this study 226 samples (80 human samples, 60 dog samples, 36 cat samples and 50 poultry samples) were examined for the presence of coagulase positive staphylococci by using conventional method and PCR. Based on conventional methods there is 32 isolates were identified coagulase positive by slide method, whereas this number was diminished by tube method to be 20 isolates. To confirm S. aurues isolates, Voges-Proskauer test was applied as simple confirmatory test for S. aurues. The test revealed 16 isolates were are full identified. Polymyxin B resistance test was used to differentiat coagulase positive staphylococci, and two isolates completelty identified as S. intermedius depending on phenotypic parameters. Thermonuclease and coagulase gene were amplified in both species S. aurues and S. intermedius with the same primer pairs. The PCR results of coagulase positive staphylococci were subjected to restriction digestion using RFLP with AluI. The banding profiles of RFLP were 4 distinct profiles (I, II, III &IV). Discrimination powers of RFLP not enough to differentiate S. aureus from S. intermedius. The sequencing for coa gene was done for selected 10 isolates including 2 S. intermedius isolates. The constructed phylogenetic trees for Staphylococcus isolates revealed a suppose of the major ancestor for animal infection cases could come from human cases. In spite of all isolates are from different origins but it’s harboring the major one genotype of S. aureus clone. It is tempting to say that coa gene sequence of S. intermedius was reported as far as it is known for the first time in Egypt on GenBank with accession No. KU178988 & KU178993. It is loped that the data obtained puts this study in perspective when designing control strategies of staphylococcal infection as the issues in the concern