الفهرس 
Title : Isolation and characterization of avian reovirus associated with arthritis in broiler breeders
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Abstract
Samples of tibiotarsal tendons, tendon sheaths and fluids around ruptured tendons were collected from broiler breeder flock of 28 weeks old in Giza governorate, showing symptoms of arthritis, lameness and swelling in the hock joints. Trial for isolation of avian reovirus (ARV) was done by inoculation in embryonated chicken eggs (ECE). After propagation and titration of the isolated virus, it had been antigenically characterized by agar gel precipitation test (AGPT), virus neutralization test (VNT) and fluorescent antibody technique (FAT). Pock lesion on the chorioallantoic membrane (CAM) was negatively stained and examined by electron microscope, also histopathological examination was carried out. Molecular characterization was performed by reverse transcriptase polymerase chain reaction (RT-PCR). The results confirm the isolation of avian reovirus from the suspected flock. The isolated virus designated as ARV/EGYPT/GIZA 2011