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Abstract Purpose: to evaluate the efficacy of low intensity laser versus polarized light therapy on serological response and polymerase chain reaction in genital herpes. Methods of evaluation (measurement of serological tests (IgG and IgM) as well as the polymerase chain reaction). Methods: Forty five patients (15 males and 30 females) suffering from genital herpes simplex infection type II and their ages ranged from 20 to 45 years. They were divided into three groups. group (A) composed of 15 patients received the LILT for 10 minutes (for the area with cutaneous manifestations) in addition to the topical acyclovir cream 5% twice / day through the treatment period. group (B) received the BLT for 10 minutes (for the area with cutaneous manifestations) in addition to the topical acyclovir cream 5% twice / day through the treatment period (day after day for 2 months). group (C) received the topical Acyclovir cream 5% twice / daily throughout the treatment period only. Results and conclusion: Results showed that application both the LILT and BLT in addition to the topical acyclovir cream 5% were effective than application of the topical acyclovir cream 5% alone in improving the genital herpes pain and cutaneous manifestations. But LILT was more beneficial than BLT in improving the genital herpes pain and cutaneous manifestations as evidenced by the highly significant decreases in IgG, IgM and PCR |