الفهرس 
Title : Association of GSTP1 (Glutathione S-transferase P1) gene polymorphism with acute lymphoblastic leukaemia ALL) in Egyptian pediatric patients
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Abstract
It was detection of GSTP1 gene mutation as a risk factor for acute lymphoblastic leukaemia in children patients and correlating the different GSTP1 genotypes.Patients & Methods One hundred pediatric patients with de novo acute lymphoblastic leukaemia who were seen at the pediatric department of National Cancer Institute, Cairo University. One hundred healthy individuals of an age and sex closely similar to those of the patients and selected from Abo Elreesh Hospital, Cairo University were included as controls. We detected the genotypes of GSTP1 codon 105 Ile{u2215}Val polymorphism using peer based restriction fragment length polymorphism (RFLP) technique. Patients subjected to complete blood picture, kidney function tests, lactate dehydrogenase enzyme, cerprospinal fluid examination, genotyping of GSTP1 Ile 105 Val polymorphism through PCR{u2013}RFLP, bone marrow aspiration, mmunophynotyping and DNA index and cytogentics (favorable and unfavourable) for patients. Results Haemoglobin (Hgb) level showed an average of 8.05 g/dL (± 0.75) and a median of 7.5 g/dL (a range between 5.5 and 11.2 g/dL). Also, total leucocytic count (TLC) had an average of 83.13 x10³/mm³ (± 7.8) and a median of 76.25 x10³/mm³ (a range between 1.65 and 480 x10³ cells /mm³). Platelet count had an average of 32.12 x10³/mm³ (± 2.8) and a median of 26.5 x10³/mm³ (a range between 10 and 90 x10³/mm³). Blasts were detected in peripheral blood in only patients not control group. Blasts had a mean of 68.5 x 10³/mm³ (65%) (± 2.4) and a median of 20.33 x 10³/mm³ (80%) (a range between 0.7 and 424 x 10³/mm³).In our study, DNA indx was measured using immunophenotyping. Hypodiploidy was diagnosed in 11 patients (44%) with DNA index less than 1.16 and was considered to have a bad prognosis. On the other hand, hyperdiploidy was diagnosed in 14 patients (56%) with DNA index equal to or more than 1.16 and was considered a good prognosis (P= 0.21)