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العنوان
Performance and oxidation status of Nile tilapia (Oreochromis niloticus) in response to some dietary and ecological modifications /
الناشر
Gehad Eisa Ahmed Mohamad Eisa ,
المؤلف
Gehad Eisa Ahmed Mohamad Eisa
هيئة الاعداد
باحث / Gehad Eisa Ahmed Mohamad Eisa
مشرف / Fathy Farouk Mohamed
مشرف / Ramadan A. El-Banna
مشرف / Ahmed M. El- Ashram
تاريخ النشر
2017
عدد الصفحات
164 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
16/10/2016
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Nutrition and Clinical Nutrition
الفهرس
Only 14 pages are availabe for public view

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Abstract

Two experimental trials were conducted. The first feeding trial was conducted to study the impact of different Methionine sources on performance and oxidation status of Nile tilapia (Oreochromis niloticus) fingerlings. A total of 180 Nile Tilapia fingerlings weighing (8.2) g were randomly assigned into four equal groups with 3 replicates (each of 15 fingerlings), reared in glass aquaria, supplied with clean de-chlorinated tap water with air pumping and filtration system applied throughout the experiment. Fish in different groups were fed on 4 experimental isocaloric, isonitrogenous purified basal diets composed of corn starch, casein, gelatine and cellulose as the main components to cover the nutrient requirements of Nile Tilapia fingerlings as described by NRC feeding standards (2011). The first group (G1) was fed on basal diet without extra Met supplementation (Met deficient), the second group (G2) was fed on the same diet with DL-Met to cover the requirements, the third group (G3) was fed on the same diets with MHA-Ca (BE 88%) supplementation and the fourth group (G4) was supplemented with MHA-FA (BE 65%) The experiment lasted for 3 months. After 2 weeks which served as acclimatization period during which the suitable feeding regime were suggested to be (4% of fish biomass). Growth performance parameters were biweekly recorded. At the end of the experiment, serum samples were collected to determine some selected serum parameters including, Total Protein, Albumin, Globulin, Albumin / Globulin ratio, ALT, AST, Uric acid and Creatinine. Also samples from the homogenates hepatic tissue were collected for determination of the oxidation stress markers including reduced Glutathione (GSH), Malondialdehyde, (MDA), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione reductase (GR) and superoxidedismutase (SOD) activity. Lysozomal activity and NBT (Respiratory Oxygen burst effect) were evaluated to determine the immune status