الفهرس 
Title : Rapid immunological assays for detection of aflatoxin B? in animal feed and its products
AbstractOnly 14 pages are availabe for public view
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Abstract
Aflatoxin B₁, a fungal secondary metabolite excreted mainly by toxigenic Aspergillus flavus, considered as the most dangerous naturally occurring toxin, has carcinogenic effect on both human and animals. Aflatoxin B₁ was successfully produced from local toxigenic Aspergillus flavus strain on YES medium and its amount was determined using HPLC. Aflatoxin B₁ is a toxic fungal metabolite of low molecular weight, and hence is devoid of antigenicity. Those toxins also lack a reactive group for coupling with a macromolecule carrier for antibody production. Therefore, the main goal of this study was to produce polyclonal antibody (IgG) against aflatoxin B₁ through immunization of New Zealand white rabbits by the prepared antigen (AF B₁-BSA Conjugate). The produced polyclonal antibodies were used in the production of a diagnostic product that was tested against spiked and naturally contaminated samples. Spiked samples were tested using both sandwich and competitive ELISA. In case of sandwich ELISA, the recovery % obtained from finished cattle feed samples ranged from 80.3% and 96% while in meat samples AF B₁ ranged from 78.1% and 93%. For competitive ELISA, the mean recovery % of AF B₁ obtained from finished cattle feed samples ranged from 88.7% and 97% , while in meat samples it ranged from 89.6% and 103%. Using competitive ELISA, The produced product gave promising results and was efficient in detecting AF B₁ in the examined naturally contaminated samples. In conclusion, the diagnostic product produced in the present study is simple and economic. ELISA procedure that can be used to screen aflatoxin B₁ in variety of samples (animal feed samples, meat and meat products). Thus, it is possible to screen large number of samples simply and inexpensively for the presence of aflatoxin B₁