الفهرس Only 14 pages are availabe for public view
 |  | from | 129 |  |  |
| | | from | 129 | | |
Abstract
Background: Tumor necrosis factor-alpha (TNF-Ü) encodes a pro-inflammatory cytokine that is secreted primarily by macrophages and plays critical roles in the pathogenesis of inflammatory autoimmune and malignant diseases. The TNF-Ü protein induces the expression of adhesion molecules, facilitating the invasion of metastatic tumor cells. Aim of the work The aim of the present study is to determine the genotypes and alleles frequencies of TNF-Ü {u2212}308 G > A polymorphisms among some of the Egyptian patients with chronic HCV infection and healthy individuals to assess whether these genes are involved in chronic HCV susceptibility and/or HCC development. Subjects and Methods: sixty chronic HCV patients selected from those admitted to the tropical department in Theodor Bilharz Research Institute were enrolled in the present study. Patients were divided into 2 groups: group A: included (30) chronic hepatitis C patients group B: included (30) patients with HCC on top of chronic hepatitis C In addition to Control Group: thirty age and sex matched healthy volunteers will be included as a control group. All patients under study were subjected to history taking, Routine laboratory investigations, Pelvi abdominal ultrasonography, triphasic Computed tomography, Estimation TNF308.2 genotyping using Real time PCR and assay of serum TNF Ü level by ELISA. Results: A statistically significant increase in serum TNF Ü value comparing control group with the HCV patients, also between and control group and HCC patients group and between HCV group and HCC group and There is no significant difference in comparing TNF Ü -308 G/A among the studied groups regarding G/G with (P = 0.1), G/A (P = 0.1) and A/A (P value = 0.3)