الفهرس 
Study of the relation between single nucleotide polymorphisms of Forkhead Box gene (FOX) and hepatocellular carcinoma in Egyptian patients
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Abstract
Hepatocellular carcinoma (HCC) constitutes a challenging health problem in Egypt due to high incidence of hepatitis C virus (HCV) infection. HCC is characterized by genetic heterogeneity that may arise from alterations at multiple genes. The transcription factor forkhead box P1 (FOXP1) has been implicated in cellular proliferation, differentiation, metabolism and longevity as well as hepatic tumorigenesis. The present study was designed to explore the association of C/A rs2687201 and C/T rs2121783 single nucleotide polymorphisms (SNPs) in FOXP1 gene with HCC susceptibility, gene expression and protein level as well as overall survival in HCV Egyptian patients. The study included 194 Egyptian patients comprising 86 patients diagnosed with HCV infection and 108 patients with HCV-related HCC in addition to 80 volunteers as controls. SNPs were studied using allelic discrimination while qRT-PCR and ELISA were performed to assess FOXP1 gene expression and protein level, respectively. The current results revealed a significant association between FOXP1 rs2687201 and HCC risk evidenced by higher A allele and (CA+AA) genotype frequencies in HCC patients compared to HCV patients and controls in a dominant model. In addition, FOXP1 rs2121783 was significantly associated with HCC risk evidenced by higher T allele and TT genotype frequencies in HCC patients in comparison with HCV patients and controls in a recessive model. However, no such association was found when comparing HCV patients to controls. Our data also showed that rs2687201 (CA+AA) and rs2121783 TT genotypes were associated with high FOXP1 gene expression and protein level. Furthermore, rs2687201 (CA+AA) genotypes displayed a significantly shorter overall survival than CC genotype in HCC patients while rs2121783 TT genotype exhibited a non-significantly shorter overall survival than (CC+CT) genotypes in HCC patients. In conclusion, FOXP1 gene polymorphisms rs2687201 and rs2121783 are significantly associated with HCC, but not with HCV infection, in Egyptian patients