الفهرس 
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Abstract
Name of candidate: Ehab Abdelaziz Aziz El-Hefny Degree: M. V. Sc.
Title of Thesis: Molecular Studies on Mange in Rabbits
Supervisors:
Prof. Dr. Nasr Mawoud Elbahy
Prof. Dr. Mahmoud Rezk Aboulaila
Prof. Dr. Ahmed Osman Elkhatam
Department of Parasitology
Branch: Parasitology
Approval:
Abstract: The present study aimed to clinical and molecular investigation of the prevalence of Sarcoptic mange in rabbits in some commercial rabbitaries in and its relation with some hematological, biochemical and histological changes in affected rabbits and molecular identification of manage infested rabbit under Egyptian conditions. A total of 1120 rabbits, located in different localities of Quesna district, Menoufiya Governorate during April (2017) to March (2018), were examined grossly to detect the incidence of abnormal dermatpathy; from which 67 rabbits had an interesting dermatological affection. So, 21 skin scrapping, serum, and PM skin samples were collected for further examinations. The results cleared that, the number of rabbit population that we act on its about 1120 rabbit, the number of rabbits that suspected to infection about 97 rabbits from them 67 rabbit were examined for mange infection by about 69.10 % .The higher incidences of mange in males showed a higher level 32 (47.80 %) and in female reached to 35 (52.20 %). The higher incidences of the mange observed in ear the higher incidences of mange observed in Male 11(16.41%) and in Female 10 (14.92 %). In male the most sites of infection includes leg, body, ear and nose. While in female , the most common sites of infection includes leg, face, nose and ear. The main clinical signs observed on infested rabbits with, sarcoptic mange is characterized by intense pruritus, alopecia, erythema, scales, and crusts around the head, neck, trunk, feet, and genitals .White blood cells, lymphocytes, liver enzymes (GOT and GPT), urea, total antioxidant capacity, glutathione peroxidase, and malondialdehyde dramatically increased whereas RBCs, Hb, and MCV significantly decreased. There were epidermal thickening and hyperkeratosis, inflammation, and homogenous faint pink edematous lesions, and the S. scabiei was attached to the stratum corneum and/or burrowing through it, causing tunnels. PCR and sequence analysis of the ITS-2 region and Cox1 and Actin genes showed that the sequences in the present study were highly identical to the homologous sequences from several countries and confirmed that the mite was S. scabiei. This study presented the first molecular characterization of S. scabiei in rabbits from Minoufiya Governorate, Egypt
Key words: Rabbits, Sarcoptes scabiei, ITS2, COX 1, Actin, Histopathology, hematology, biochemical, skin scraping