الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
مستخلص الرسالة باللغة الانجلBC is the most frequent cancer among women. It is also the world’s second biggest
cause of cancer mortality. According to malignancy statistics 2020, BC accounts for 30% of
all female malignancies, with 276,480 new BC and over 42,000 mortality expected in 2020.
In Egypt, it accounts for 33% of all female cancer cases, with over 22,000 new cases
identified each year. Many cancers are treated with radiotherapy, but radio resistance is still a
major factor in radiotherapy failure. Radiotherapy causes oxidative damage in all cellular
compartments, including the lipid membrane, on a random basis. Oxidative stress
accumulation has been linked to the direct cause of pyroptosis, Pyroptosis is a newfound
inflammatory programmed cell death. Membrane perforation, cell swelling and cell rupture
are crucial characteristics of pyroptosis (Lei et al. 2020).
The objective of this study was to study pyroptosis before and after RT in BC patients
based on the serum levels of GSDMD-CT, NLRP3 IL-18.
This research included 70 subjects divided into two main groups: control group : The
control group consisted of 30 healthy volunteers who were age and sex matched. group II:.
40 BC patients treated with RT, The dose given was a 2.75 Gray daily irradiation dose, for
five weeks, five days a week, 44 Gy total dose. Patients were selected from those admitted to
Bahia hospital, Cairo, Egypt.
Serum was separated and Circulating pyroptosis biomarkers IL-18, NLRP3 and
GSDMD-CT levels were assessed by using ELISA technique.
Our results showed that:
Statistically significant inecrease in serum NLRP3 in BC patients after RT when
compared to before radiotherapy and statistically significant difference between BC
patients before and after RT treatment when compared to control group.
Statistically significant increase in serum GASDERMIN in BC patients after RT when
compared to before RT and statistically significant difference between BC patients
before and after RT treatment when compared to control group.
Statistically significant increase in IL18 in BC patients after irradiation when compared
to before RT and statistically significant difference between BC patients before and
after RT treatment when compared to control group.
6.2. Conclusion
Our conclusions can be outlined as:
- Radiotherapy induced pyroptosis in breast cancer patients as a new cell death
mechanism.
- Gasdermin, NLRP3 and IL18 is biomarkers of pyroptosis.
- Serum Gasdermin, NLRP3 and IL18 significantly increased post irradiation highlighting
enhanced ROS and pyroptotic induction.
Summary, Conclusion and Recommendations
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- Pyroptosis could be targeted in the therapy of BC patients.
6.3. Recommendations
from the above findings, we may recommend the following:
- Gasdermin, NLRP3 and IL18 measuring should be included in the work-up of patients
subjected to radiotherapy prior to and after termination of the sessions being markers of
radiotherapy efficacy