الفهرس | Only 14 pages are availabe for public view |
Abstract Food is a good source of protein which makes it a good substrate for microbial growth. In this study, the prevalence, antibiogram, virulence and antimicrobial resistance genes of some foodborne pathogens from airport catering and the surrounding area meals provided to passengers were evaluated. Therefore, 440 samples (220 raw and 220 cooked samples) samples, including minced meat (50), cubes (100), chicken breast (100), chicken shish (50), salmon fish (70), and Nile Perch (70) were collected from Egyptian airport caterin and surrounding resturants. Samples were subjected to bacteriological evaluation for S. aureus, Escherichia coli and Salmonella. The results of the current study revealed that The bacteriological examination of 440 samples, including minced meat (50), cubes (100), chicken breast (100), chicken shish (50), salmon fish (70), and Nile Perch (70) were collected from Egyptian airport catering, Egypt. Out of 440 samples 25 (5.6%) samples were positive for bacterial isolation including S. aureus 13 (2.9%), E. coli 10 (2.3%) and Salmonella 2 (0.45%). VITEK2 software data was compared to the biochemical test results to estimate the findings. The VITEK2 compact equipment and the application of GN cards confirmed the identification of all isolates recovered, with a probability of 100%. Biochemical identification and VITEK2 produced the same results. Recovered samples of E. coli Author Identification ii were tested to detect eaeA , stx2 and stx1 as virulence genes and ESBL (blaTEM, blaCTXM and blaSHV), ampC genes (blaCMY, blaDHA), Quinolones (qnrA), Aminoglycoside (aadA1), Tetracycline (tetA) and Sulfonamide (sul1) as antimicrobial resistance genes. Recovered samples of Salmonella were tested to detect stn (Salmonella enterotoxin), hilA (gene encodes an ompR/ToxR family transcriptional regulator) and pefA (plasmid encoded fimbriae) as virulence genes and ESBL (blaTEM,blaCTX-M and blaSHV), ampC genes (blaCMY,blaDHA), Quinolones (qnrA), Aminoglycoside (aadA1 strA (Streptomycin), Tetracycline (tetA) and Sulfonamide (sul1) as antimicrobial resistance genes. In conclusion, good manufacturing practices with well-trained food handlers are necessary to control and prevent contamination of the airport meals by foodborne bacteria. |