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العنوان
Molecular Studies on Some Food
Poisoning Bacteria Isolated from
Airport Catering /
المؤلف
Bolbol, Mohamed Hassan Mohamed.
هيئة الاعداد
باحث / محمد حسن محمد بلبل
مشرف / محمود عزت السيد
مناقش / ايمان خليفة صديق
مناقش / محمد السيد عناني
الموضوع
Salmonella. Foodborne.
تاريخ النشر
2022.
عدد الصفحات
134 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
26/9/2022
مكان الإجازة
جامعة قناة السويس - كلية الطب البيطري - البكتريا والمناعه والفطريات
الفهرس
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Abstract

Food is a good source of protein which makes it a good substrate for microbial growth.
In this study, the prevalence, antibiogram, virulence and antimicrobial resistance genes
of some foodborne pathogens from airport catering and the surrounding area meals
provided to passengers were evaluated. Therefore, 440 samples (220 raw and 220
cooked samples) samples, including minced meat (50), cubes (100), chicken breast
(100), chicken shish (50), salmon fish (70), and Nile Perch (70) were collected from
Egyptian airport caterin and surrounding resturants. Samples were subjected to
bacteriological evaluation for S. aureus, Escherichia coli and Salmonella. The results
of the current study revealed that The bacteriological examination of 440 samples,
including minced meat (50), cubes (100), chicken breast (100), chicken shish (50),
salmon fish (70), and Nile Perch (70) were collected from Egyptian airport catering,
Egypt. Out of 440 samples 25 (5.6%) samples were positive for bacterial isolation
including S. aureus 13 (2.9%), E. coli 10 (2.3%) and Salmonella 2 (0.45%). VITEK2
software data was compared to the biochemical test results to estimate the findings.
The VITEK2 compact equipment and the application of GN cards confirmed the
identification of all isolates recovered, with a probability of 100%. Biochemical
identification and VITEK2 produced the same results. Recovered samples of E. coli
Author Identification
ii
were tested to detect eaeA , stx2 and stx1 as virulence genes and ESBL (blaTEM, blaCTXM and blaSHV), ampC genes (blaCMY, blaDHA), Quinolones (qnrA), Aminoglycoside
(aadA1), Tetracycline (tetA) and Sulfonamide (sul1) as antimicrobial resistance genes.
Recovered samples of Salmonella were tested to detect stn (Salmonella enterotoxin),
hilA (gene encodes an ompR/ToxR family transcriptional regulator) and pefA (plasmid
encoded fimbriae) as virulence genes and ESBL (blaTEM,blaCTX-M and blaSHV), ampC
genes (blaCMY,blaDHA), Quinolones (qnrA), Aminoglycoside (aadA1 strA
(Streptomycin), Tetracycline (tetA) and Sulfonamide (sul1) as antimicrobial resistance
genes.
In conclusion, good manufacturing practices with well-trained food handlers are
necessary to control and prevent contamination of the airport meals by foodborne
bacteria.