الفهرس 
Production of callus from different parts of Balanites Aegyptiacae and study the effect of callus extract on different cell lines
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Abstract
the aim of this study to evaluate the role of plant growth regulator (PGR) and different
elicitors in vitro to induce the enhancement of bioactive compound (Diosgenin) production in callus culture
from different parts of Balanites Aegyptiacae plant and study the effect of diosgenin extract free and
functionalized with IONPs synthesized by coprecipitation method on cell viability of HepG carcinoma and
A lung carcinoma and determination of P levels in HepG . Results and conclusion: the highest
diosgenin content obtained from root part culture elicited with tryptophan mg/l was mg/ g DW, the
cytotoxic activity of the root callus extract supplemented with tryptophan was determined against HepG (hepatic
carcinoma), A (Lung carcinoma cell lines) and the result was μm/ml, μm/ml respectively. Also the
results of this study showed that the FTIR and HRTEM confirm the formation of IONPs with size ranging from ( -
)nm , FTIR confirm the conjugation chemistry between diosgenin and IONPs via citric acid as a linker by the
presence of iron oxide signature peak at cm- and signature peak of diosgenin at , and
cm- . also our results demonstrated that the cytotoxic effect of functionalized diosgenin IONPs-D
increased against HepG and A and became IC at μg and μg respectively , Our study
reported that Diosgenin free and functionalized induced cell cycle arrest and apoptosis via activation of P
expression to be and respectively when compared with control.