الفهرس 
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Abstract
from July to August 2017, private farm in Egypt’s Dakranis Dakahlia Governorate served as the place for the experimental work for this study. The current study’s objective would have been to study at whether broiler chicken growth performance and certain blood metabolites will be affected by delaying feeding and supplementing with high levels of important amino acids.In this study, 160 broiler chicks one-day old of Ross 308, unsexed broiler chicks were placed into 5 groups, each of which had 4 replicates (floor pens) each replicate content 8 broiler chickens. During experimental procedures, a diet based on corn and soybean meal was give as a control (T1) containing 21 CP% and 3000 k Cal/kg of ME supplemented with 100% essential amino acid contents recommended by NRC (1994), the second treatment was fed the basal diet after fasting for 24 h (T2), the third treatment was fed the basal diet supplemented with high level of lysine 12.9 g/kg (T3), the fourth treatment was fed the basal diet including lysine, methionine and threonine requirements of 13.7, 6.38 and 8.9 g/kg of diet, respectively (T4). The fifth treatment was fed the basal diet including lysine, methionine, threonine, and valine requirements of 14.7, 6.8, 9.9, and10.4 g/kg of diet, respectively (T5). These birds were fed the starter diet until 21 days of age, and then the experimental birds were fed on one growing ration until 35 days of age. Each pen’s length and width were 70 and 70 cm, respectively, and it was used to raise birds. The pen’s floor thus covered 0.49 m2 (70 x 70 cm). The starter diet was given to chickens from one day old to 21 days old, and a grower ration was given to them from 22 days old to 35 days old. 5.1. Growth Performance of Broiler Chicks: 5.1.1. Live Body Weight:Live body weight was found non-significant (P<0.05) between the experimental groups of broiler chicks from 0 to 14 days but the treatments of amino acid -supplementation were highest weight compared with the T1 and T2. In the 3rd and final live body weight there were a significant difference between the control group (T1), fed fasting 24 h after hatching (T2) and the other treatments which amino acid- supplemented and the highest live body weight (803 and 2957 g, respectively) were observed in the treatment (T5) amino acid-supplemented with Lysine, Methionine, Threonine, and valine requirements of 14.7, 6.8, 9.9, and 10.4 g/kg of diet.5.1.2. Body Weight Gain: Supplementing with dietary amino acids had no discernible effect on BWG. (P<0.05) during the periods from 0 to 21 day however, the treatment (T5) who extra amino acid from lysine, methionine, threonine, and valine the better in BWG as compared to all other groups. Whereas in during the periods from 22 to 35 day a significant difference was observed in BWG of broilers amino acid-supplemented compared with the control and fasting treatments (P<0.05). Whereas the best broiler chickens fed the dietary extra amino acid from lysine, methionine, threonine, and valine (T5).5.1.3. Feed Intake: The impact of feeding broiler chicks’ diets enriched with amino acids over the entire study period on feed consumption. Between the control group fed on the basal diet or group fed the basal diet after fasting 24 h, and the other groups supplemented with dietary amino acids, there were significant differences in the feed intake of broiler chicks aged 0 to 35 days (P<0.05). Moreover, broilers fed the diet fortified with extra amino acid from lysine, methionine, threonine, and valine (T5) significantly enhanced FI than the other experimental groups during whole experimental periods. 5.1.4. Feed Conversion Ratio:The feed conversion ratio of broiler chicks did not substantially differ between the control group and the other treatments from 0 to 35 days of age. But broilers fed the diet fortified with high level of lysine 12.9 g/kg (T3) displayed significantly better FCR (1.6 feed: gain) than the other experimental during the period from 7 to 14 days of age.5.2. Blood Constituents and Immune Response: Dietary supplementation with amino acid- supplementation (lysine, methionine, threonine, and valine) these no significant different the plasma levels total protein, albumin, AST, uric acid, T3, T4, IgM and IgG. But the experimental groups for amino acid- supplementation and group fed basal diet after fasting 24 h were higher concentration for ALT compared with control group. 5.3. CONCLUSION: According to a recent study on broiler production, chicken performance was enhanced by starting diets with less CP and additional doses of essential amino acids such lysine, methionine, threonine, and valine during the start period. On the other hand, increasing the amount of dietary amino acids has no appreciable effect on the blood measurements in broiler chickens fed low protein diets.